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KMID : 1098420230310060414
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Korean Journal of Medicinal Crop Science
2023 Volume.31 No. 6 p.414 ~ p.423
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Changes in Content of Luteolin Derivatives in Humulus japonicus Extracts Prepared using Different Drying Methods
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Lee Kyoung-In
Pyo Byoung-Sik
Choi Chul-Hee
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Abstract
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Background: The main compounds in the Humulus japonicus extract prepared by drying for 12 h under hot air at 50℃ (50-12H), or 7 d, at room temperature at 25℃ (25-7D) were analyzed.
Methods and Results: Three main compound peaks were identified in liquid chromatograph anal- ysis using an ultraviolet detector. Additionally, mass spectrometer analysis was performed, and each peak was confirmed to either belong to luteolin 7-O-glucoside, luteolin 4'-O-glucoside, or luteolin through standard analysis. The 50-12H extract showed high contents of luteolin 7-O-glu- coside and luteolin 4’-O-glucoside. Meanwhile, in the 25-7D extract, the luteolin 7-O-glucoside and luteolin 4’-O-glucoside contents reduced and the luteolin content increased, this is because luteolin 7-O-glucoside and luteolin 4’-O-glucoside decomposed into luteolin and glucose by the action of hydrolytic enzymes. In other words, a relatively slow drying speed provides enough time for hydrolytic enzyme to be activiated in H. japonicus, which increases the luteolin content.
Conclusions: Room temperature drying is suitable for increasing the luteolin content in H. japoni- cus extract, and a fast heat drying method is suitable for increasing the luteolin glycoside content.
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KEYWORD
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Humulus japonicus, Liquid Chromatography, Luteolin Derivative, Mass Spectroscopy
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